Transforming Small Interfering RNA (siRNA) Bioanalysis

trasiR™ for reliable siRNA quantification made easier

Extraction-free

Works directly in complex biological media

Eliminate noise from varying extraction efficiencies.

Simplified and rapid workflow

Fast and simple workflow

Minimal protocol steps and measurement on a fluorescence plate-reader.

Optimised for small interfering RNA

Built for siRNA

Overcomes limitations of other techniques from unique challenges posed by siRNA.

Compatible with chemical modifications

Suits a wide variety of chemical modifications.

Including standard Phosphorothioate (PS), 2’-O-Methoxyethyl (MOE), 2’-O-Methyl (OMe) modifications.

Introducing trasiR™ for pharmacokinetic bioanalysis of small interfering RNA

trasiR™ offers quantification Small Interfering RNA Therapies (siRNA) directly in biological samples such as blood serum. Using Nanovery's patented Nucleic Acid Nanorobotics (NANs) platform, trasiR™ comes as a reagent, offering an enzyme-free and isothermal assay format designed for simplicity and reliability.

trasiR™ can be easily tailored to support a range of ADME, biodistribution and pharmacokinetic (PK) studies in bioanalysis, with the flexibility to accommodate various siRNA patterns, chemical modifications, chemical conjugates and sample types.

trasiR™ compared to standard quantification techniques for siRNA

Solid-phase extraction LC-MS
LC-MS (SPE)
Hybrid LC-MS
LC-MS (Hybrid)
hELISA
hELISA
SL-RT-qPCR
qPCR
trasiR™
trasiR™
Sensitivity
Sensitivity
Low
(10–20 ng/ml)
Sensitivity
Medium
(0.5–25 ng/ml)
Sensitivity
Medium
(0.3–4 ng/ml)
Sensitivity
High
(0.007 ng/ml)
Sensitivity
High
(~10 pM)
Pros
Pros

Specific metabolite detection
No custom reagents needed

Pros

Specific metabolite detection

Pros

High throughput

Pros

High sensitivity

Pros

Direct to sample

Simple protocol

Robust and reproducible

Low working volume

Cons
Cons

Chromatography

Poor sensitivity

Ruggedness

Low throughput

Cons

Probe design

Chromatography

Low throughput

Cons

Probe design

Specificity

Analyte specific probes needed

Long assay development time

Cons

Laborious protocol

Primer design

Specificity

Cons

Experiencing these issues in PK assays for antisense oligonucleotides?

Chemical modifications

siRNA are chemically modified to boost their stability, uptake and target affinity. These modifications, including additional moieties for stability, targeting, and biodistribution, also interfere with the efficiency of enzymatic processes in qPCR, leading to inconsistencies in quantification.

trasiR™ assay is based on our proprietary NAN technology. It is distinctively enzyme-free and relies on nucleic acid hybridisation and strand displacement reactions, which makes it excel at consistently detecting siRNA. Read more about our technology (here).

RNA extraction efficiency

Long assay development times

Complex and Costly Equipment and protocols

Speak with our siRNA Specialist

Ennio will reach out to discuss your project’s specific needs.

Proven performance of trasiR™ according to ICH-M10 guidelines

Our DNA nanorobots demonstrate remarkable compatibility with chemical modifications, offering robust detection and precise quantification of antisense oligonucleotides. We validate the siRNA detection and quantification assay in line with ICH-M10 guidelines to guarantee high levels of accuracy, precision, and selectivity across biological matrices. You can find our full collection of validation data here.

Compatibility with chemical modifications in antisense therapies

The results below illustrate the robust detection capabilities of NANs across four sample types: 1) DNA, 2) RNA, and siRNA with 3) OMe, and 4) MOE modifications. These assays demonstrate excellent linear responses, enabling precise quantification within the low picomolar range. Small error bars indicate high accuracy, and the simplicity of data analysis highlights the practical efficacy of our technology in various bioanalytical settings.

DNA chart
RNA chart
OMe chart
MOE chart

Order custom bioanalytical assay for your siRNA

  1. Discuss your project requirements with our expert team.
  2. We will evaluate your siRNA's characteristics.
  3. Our team will prepare a proposal tailored to meet your needs.
  4. Get a complimentary evaluation license with every assay.

Interested in how we can support your siRNA pipeline?