Antisense Oligonucleotide (ASO) Analysis

Transforming Antisense Oligonucleotide (ASO) Bioanalysis

trASO™ for reliable ASO quantification made easier

Extraction-free

Works directly in complex biological media

Eliminate noise from varying extraction efficiencies.

Simplified and rapid workflow

Fast and simple workflow

Minimal protocol steps and measurement on a fluorescence plate-reader.

Optimised for short antisense oligonucleotides

Built for ASOs

Overcomes limitations of other techniques from unique challenges posed by ASOs.

Compatible with chemical modifications

Suits a wide variety of chemical modifications.

Including standard Phosphorothioate (PS), 2’-O-Methoxyethyl (MOE), 2’-O-Methyl (OMe) modifications.

Introducing trASO™

trASO™ offers quantification Antisense Oligonucleotide Therapies (ASOs) directly in biological samples such as blood serum. Developed using Nanovery's patented Nucleic Acid Nanorobotics (NANs) platform, trASO™ comes in the form of a reagent, offering an enzyme-free and isothermal assay format designed for simplicity and reliability.

trASO™ can be easily tailored to support a range of ADME, biodistribution and pharmacokinetic (PK) studies in bioanalysis, with the flexibility to accommodate various ASO patterns (such as Antagomir, Gapmer, ssDNA), chemical modifications, and sample types.

Types of Antisense Oligonucleotides (ASOs)

trASO™ compared to standard quantification techniques

LC-MS
LC-MS
hELISA
hELISA
Branched DNA
Branched DNA
qPCR
qPCR
trASO™
trASO™
Sensitivity
Sensitivity
Low
(~1 nM)
Sensitivity
Medium
(~100 pM)
Sensitivity
High
(~10 pM)
Sensitivity
High
(~10 pM)
Sensitivity
High
(~10 pM)
Pros
Pros

Specific metabolite detection

Dynamic range

Pros

High throughput

Pros

Direct to sample

Pros

Low sample volume required

Pros

Direct to sample

Simple protocol

Robust and reproducible

Low working volume

Cons
Cons

Poor sensitivity

High cost

Requires re-optimisation between projects/targets

Cons

Poor specificity

Robustness and reproducibility issues

Cons

Low throughput

Multiple washing steps

Robustness and reproducibility

Cons

Laborious purification protocol

Requires splint ligation reaction and extensive optimisation

Cons

Experiencing these issues in PK assays for antisense oligonucleotides?

Chemical modifications

ASOs are chemically modified to boost their stability, uptake and target affinity. These modifications, including additional moieties for stability, targeting, and biodistribution, also interfere with the efficiency of enzymatic processes in qPCR, leading to inconsistencies in quantification.

trASO™ assay is based on our proprietary NAN technology. It is distinctively enzyme-free and relies on nucleic acid hybridisation and strand displacement reactions, which makes it excel at consistently detecting ASOs. Read more about our technology (here).

RNA extraction efficiency

Long assay development times

Complex and Costly Equipment and protocols

Speak with our ASO Specialist

Alexander will reach out to discuss your project’s specific needs.

Our results

Our DNA nanorobots demonstrate remarkable compatibility with chemical modifications, offering robust detection and precise quantification of antisense oligonucleotides. We validate the ASO detection and quantification assay in line with ICH-M10 guidelines to guarantee high levels of accuracy, precision, and selectivity across biological matrices. You can find our full collection of validation data here.

Proven performance of trASO™

The results below illustrate the robust detection capabilities of NANs across four sample types: 1) DNA, 2) RNA, and ASOs with 3) OMe, and 4) MOE modifications. These assays demonstrate excellent linear responses, enabling precise quantification within the low picomolar range. Small error bars indicate high accuracy, and the simplicity of data analysis highlights the practical efficacy of our technology in various bioanalytical settings.

DNA chart
RNA chart
OMe chart
MOE chart

Order custom ASO assay

  1. Discuss your project requirements with our expert team.
  2. We will evaluate your ASO's characteristics.
  3. Our team will prepare a proposal tailored to meet your needs.
  4. Get a complimentary evaluation license with every assay we develop.
trASO Assay Data Sheet

Download Full Product Features

Discover the precision and efficiency of the trASO™ assay with our detailed specs and case studies.

Learn how our technology is transforming ASO bioanalysis, offering a significant leap in simplicity and performance over alternative methods. Access our recent materials now to unlock the full potential of your research and development.

Explore more or contact us for details